AUTHOR=Lê Kim-Anne , Li Yan , Xu Xiaojin , Liu Tingting , Yang Wanting , He Fang , Su Kuanyuan , Cai De Hong , Go Vay Liang W , Pandol Stephen , Hui Hongxiang 

TITLE=Alterations in fecal Lactobacillus and Bifidobacterium species in type 2 diabetic patients in Southern China population

JOURNAL=Frontiers in Physiology

VOLUME=3

YEAR=2013

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2012.00496

DOI=10.3389/fphys.2012.00496

ISSN=1664-042X

ABSTRACT=<p><bold>Background:</bold> The connection between gut microbiota and metabolism and its role in the pathogenesis of diabetes are increasingly recognized. The objective of this study was to quantitatively measure <italic>Bifidobacterium</italic> and <italic>Lactobacillus</italic> species, members of commensal bacteria found in human gut, in type 2 diabetic patients (T2D) patients from Southern China.</p><p><bold>Methods:</bold> Fifty patients with T2D and thirty control individuals of similar body mass index (BMI) were recruited from Southern China. T2D and control subjects were confirmed with both oral glucose tolerance test (OGTT) and HbA<sub>1c</sub> measurements. <italic>Bifidobacterium</italic> and <italic>Lactobacillus</italic> species in feces were measured by real-time quantitative PCR. Data were analyzed with STATA 11.0 statistical software.</p><p><bold>Results:</bold> In comparison to control subjects T2D patients had significantly more total <italic>Lactobacillus</italic> (+18%), <italic>L. bugaricus</italic> (+13%), <italic>L. rhamnosum</italic> (+37%) and <italic>L. acidophilus</italic> (+48%) (<italic>P</italic> &lt; 0.05). In contrast, T2D patients had less amounts of total <italic>Bifidobacteria</italic> (−7%) and <italic>B. adolescentis</italic> (−12%) (<italic>P</italic> &lt; 0.05). Cluster analysis showed that gut microbiota pattern of T2D patients is characterized by greater numbers of <italic>L. rhamnosus</italic> and <italic>L. acidophillus</italic>, together with lesser numbers of <italic>B. adolescentis</italic> (<italic>P</italic> &lt; 0.05).</p><p><bold>Conclusion:</bold> The gut microflora in T2D patients is characterized by greater numbers of <italic>Lactobacillus</italic> and lesser numbers of <italic>Bifidobacterium</italic> species.</p>